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Event-driven high-performance cloud computing for CRISPR-Cas9 guide RNA design
DescriptionCRISPR-Cas9 has become the gold-standard technology for gene editing due to its simplicity and low cost. By carefully designing the “guide RNA” (gRNA) that directs it to the desired target site, nearly any DNA site can be edited. However, selecting good quality gRNA is a complex task and can only be achieved with high-performance computers, which are valuable resources that not all are equipped with. Here, we describe our use of event-driven cloud computing technologies made available by Amazon Web Services to overcome challenges faced by traditional standalone software for designing gRNA. Using a cloud-native software template, any researcher can deploy our software, named Crackling-Cloud, to their own cloud environment. This enables them to bring the software to their data, rather than sending their data to the software. Crackling-Cloud is available for free on GitHub under the terms of the BSD 3-clause licence: https://github.com/bmds-lab/Crackling-AWS
Event Type
Workshop
TimeMonday, 18 November 20242:21pm - 2:24pm EST
LocationB309
Tags
Broader Engagement
HPC in Society
Inclusivity
Registration Categories
W